Microenvironment has been a usually utilized term in the malignancy research field as of late. The microenvironment of a tumor is a fundamental piece of its physiology, design, and capacity. It is a fundamental piece of the tumor, since it supplies a supporting condition for the threatening interaction. Checking the tumor microenvironment by means of sub-atomic and cell profiles would be essential for recognizing cell or protein focuses for disease avoidance and treatment .Thusly, for tissue recovery in vivo the microenvironment is essentially significant. As well as checking the microenvironment of the actual tissue, observing of exogenous substances in the tissue or cell level would be fundamental. One late anticancer medication study exhibits that Nano molar groupings of arginylglycylaspartic corrosive mimetic αvβ3 and αvβ5 inhibitors can amazingly invigorate tumor development and tumor angiogenesis . In the recovery cycle of harmed tissue after injury, the recuperation or recovery is affected fundamentally by nearby climate changes in a tiny and quick scale. In the event that we have explicit and delicate instruments to screen the climate in a micro scale and constant mode, we can analyze or foresee and control the microenvironment, then; at that point we can work on the climate for effective recuperation and reclamation of harmed tissue. We as of now have an assortment of medications or apparatuses that can regulate the cell or tissue reactions despite the fact that they are typically utilized in a macro environment. There are numerous elements directing the microenvironment, which incorporates particles or electrolytes, temperature, pH, oxygen, carbon dioxide, metabolites, development components or peptides, chemicals, substantial metals, extending or contracting powers, vibration, and numerous different variables. Accordingly, we need different detecting frameworks to decisively recognize these marvels in the microenvironment. Numerous infection pathologies can be perceived through the explanation of restricted bimolecular organizations, or microenvironments. To this end, enzymatic nearness naming stages are extensively applied for planning the more extensive spatial connections in subcellular designs. In any case, advances that can plan microenvironments with higher exactness have for quite some time been looked for. Here, we depict a microenvironment-planning stage that abuses photo catalytic carbine age to specifically distinguish protein-protein collaborations on cell layers, a methodology we term Micro Map (map). By utilizing a photo catalyst-neutralizer form to spatially restrict carbine age, we exhibit specific naming of immunizer restricting targets and their microenvironment protein neighbors. This strategy recognized the constituent proteins of the modified passing ligand 1 (PD-L1) microenvironment in live lymphocytes and specifically named inside an Immunosynaptic intersection.