Background: Sexually transmitted diseases are epidemics and causes health and economic consequences in developing countries. HIV, Syphilis and other STDs are transmissible to the unborn babies and results in adverse pregnancy outcomes. This study aimed to determine the prevalence and risk factors of HIV and Syphilis among pregnant women in Ado Local Government Area, Ekiti State, Nigeria. Methods: A cross-sectional study was conducted between February and May, 2017 among 340 pregnant women attending ANC and maternity homes. Data was collected using semi-structured interviewer administered questionnaire and blood samples from enrollees were screened for HIV and Syphilis using enzyme linked Immunosorbent assay. Descriptive, bivariate and multivariate analyses were done and level of significant set at 5%.